Fluorescent Dye Wavelength Index Table

Fluorescent dyes are widely used in various scientific fields, especially in molecular biology, biochemistry, and medical diagnostics. They are molecules that absorb specific wavelengths of light and emit longer wavelengths of light. This property is used in techniques such as fluorescence microscopy, flow cytometry, and molecular fluorescent labeling.

• Fluorescence Microscopy: To visualize specific proteins, organelles, or other structures within cells.
• Flow Cytometry: To analyze the physical and chemical characteristics of cells or particles in a fluid as they pass through a laser.
• Fluorescent Labeling: To tag molecules for detection in various assays.

Creating a fluorescent dye index based on the excitation and emission wavelengths of a fluorescent dye is useful in selecting the right dye for various biological and chemical applications. Below is a basic index of some commonly used fluorescent dyes and their excitation and emission wavelengths:

Fluorescent Dye	Solvent	λex, max (nm)	λem, max (nm)	Additional Conditions for Fluorescence	λex, max (nm)	λem, max (nm)
Acriflavine	EtOH	463	490
2-Aminoacridone	pH 8.0	420	538
7-Amino-4-methyl-3-coumarinylacetic acid	MeOH	350	433
8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt	pH 7.0	356	512
8-Aminopyrene-1,3,6-trisulfonic acid trisodium salt	pH 7.4	420	500
9-(Chloromethyl)anthracene				Carboxylic acid adducts	365	412
8-Anilino-1-naphthalenesulfonic acid ammonium salt	MeOH	372	480	Protein adduct	372	480
Anthracene	CH3CN	251	401
9-Anthracenecarbonyl cyanide	MeOH	361	460	Hydroxyl compound adduct with ethanol	361	451
9,10-Anthracenediyl-bis(methylene)dimalonic acid	pH 7.0	380	407
2-(4-Biphenylyl)-6-phenylbenzoxazole	H2O		425	CH3CN, MeOH		390
1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid	pH 8.0	287	363	with calcium	274	363
1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid tetrasodium salt	pH 8.0	254	363	with calcium	274	363
bisBenzimide H 33258	pH 7.0	338	505	bound to DNA	356	465
bisBenzimide H 33342 trihydrochloride	pH 7.0	340	510	bound to DNA	355	465
2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester				Reaction product	482	528
meso-1,2-Bis(4-methoxyphenyl)ethylenediamine				Saccharide adducts	340	460
1,4-Bis(2-methylstyryl)benzene	Cyclohexane		419	Heptane		416
Bromobimane	pH 7.0	398		Thiol adduct	390	478
9-(2-Carboxy-2-cyanovinyl)julolidine	pH 8.0	433	500
5(6)-Carboxy-2',7'-dichlorofluorescein	pH 8.0	504	529
5(6)-Carboxy-2',7'-dichlorofluorescein diacetate				Reaction product	470	504
5-Carboxyfluorescein	pH 8.0	492	517
5(6)-Carboxyfluorescein	pH > 8.0	490	520
5(6)-Carboxyfluorescein diacetate				hydrolyzed	492	517
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester	DMF	<300		hydrolyzed	492	517
5(6)-Carboxyfluorescein N-hydroxysuccinimide ester	pH 9.0	495	519
5-Carboxyfluorescein N-succinimidyl ester	pH 8.0	492	517
5(6)-Carboxy-X-rhodamine	MeOH	568	595
5-Carboxy-X-rhodamine N-succinimidyl ester	pH 7.0	575	600
5(6)-Carboxytetramethylrhodamine	MeOH	541	566
6-Carboxytetramethylrhodamine	pH 8.0	543	575
5-Carboxy-tetramethylrhodamine N-succinimidyl ester	pH 7.0	543	575
5(6)-Carboxytetramethylrhodamine N-succinimidyl ester	MeOH	540	565
6-Carboxy-tetramethylrhodamine N-succinimidyl ester	pH 7.0	543	575
4-Chloro-7-nitrobenzofurazan	MeOH	336		Aliphatic amine adducts	475	540
3-(Dansylamino)phenylboronic acid	MeOH	337	517	Protein adduct	337	490
Dansylcadaverine	MeOH	335	518	Reaction product	338	500
Dansyl chloride	CHCl3	372		Butylamine adduct	337	492
Dansylhydrazine	MeOH	336	534	Saccharide adducts	336	487
4',6-Diamidino-2-phenylindole dihydrochloride	H2O			bound to DNA	358	461
Dibromobimane	MeOH	391		Thiol adduct	391	480
9-(2,2-Dicyanovinyl)julolidine	pH 8.0	433	500
7-(Diethylamino)coumarin-3-carbohydrazide	MeOH	420	468	Carboxylic acid adduct	415	480
7-(Diethylamino)coumarin-3-carbonyl azide	MeOH	432	480
7-(Diethylamino)coumarin-3-carboxylic acid	pH 9.0	409	473
7-(Diethylamino)coumarin-3-carboxylic acid N-succinimidyl ester	MeOH	432	472
3,3'-Diheptylthiacarbocyanine iodide	MeOH	562	575
Dihydroethidium	MeCN	355	420	bound to DNA	358	461
6,8-Dihydroxy-1,3-pyrenedisulfonic acid disodium salt	pH 4.0	405	465	pH 8.0	458	500
11-[5-(Dimethylamino)-1-naphthalenesulfonylamino]undecanoic acid	MeOH	335	519
4-Dimethylamino-4'-nitrostilbene	CH2Cl2	500	700
N,N-Dimethyl-6-propionyl-2-naphthylamine	MeOH	361	498
Dimidium bromide	pH 7.6, low ionic strength	480		Nucleic acid adducts, Tris pH 8.0	306	612
1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate	MeOH	550	565
3,3'-Dipropylthiadicarbocyanine iodide	MeOH	651	675
6-Dodecanoyl-N,N-dimethyl-2-naphthylamine	MeOH	364	497
Doxorubicin hydrochloride	EtOH	470	585
Eosin 5-isothiocyanate	pH 9.0	521	544
Ethidium bromide solution	H2O	480	620	bound to DNA	518	605
Ethidium homodimer	H2O	493		bound to DNA	528	617
1-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide	H2O	355	460
8-Ethoxy-N,N,N',N',N'',N''-hexamethylpyrene-1,3,6-trisulfonamide				Reaction product	380	520
Fluo-3	pH > 6.0	503		with calcium	506	526
9-Fluorenylmethyl carbazate	MeOH	264	309	Saccharide adducts	270	320
Fluorescein	pH 9.0	490	514
Fluorescein sodium salt	pH 9.0	490	514
6-[Fluorescein-5(6)-carboxamido]hexanoic acid	pH 9.0	480	525
6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester	pH 8.2	491	515
Fluorescein dilaurate				Reaction product	490	514
Fluorescein 5(6)-isothiocyanate	pH 9.0	495	520
Fluorescein isothiocyanate–dextran 150000	pH 8.0	490	520
Fluorescein isothiocyanate–dextran 70000	pH 8.0	490	520
Fluorescein isothiocyanate – Dextran 500000-Conjugate	pH 8.0	490	520
Fluorescein-5-thiosemicarbazide	pH 9.0	492	516
Fluorescent red 630	pH 7.0	620	650
4-Fluoro-7-nitrobenzofurazan	EtOH	470	528	Amino acid adducts	470	530
Fluorescamine	pH 9.0	234		Protein adduct	334	455
N-Hexadecylpyrene-1-sulfonamide	MeOH	350	400
7-Hydroxycoumarin-3-carboxylic acid	pH 4.0	342	447	pH 9.0	386	448
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester	MeOH	418	447
7-Hydroxy-4-methyl-3-coumarinylacetic acid	pH 10.0	360	445
7-Hydroxy-4-methyl-2(1H)-quinolone	pH 5.0	321	357	pH 9.0	351	438
7-Hydroxy-N-octadecylcoumarin-3-carboxamide	CHCl3/DMSO	359	406
Indo 1	100 mM KCl	349	482	with calcium	331	398
7-Methoxycoumarin	CHCl3	340	389	Reaction product	360	449
7-Methoxycoumarin-3-carboxylic acid	MeOH	360	415
2-Methoxy-2,4-diphenyl-3(2H)-furanone				Amine adducts	390	480
1-Methylpyrene	CHCl3	340	486
2'-(4-Methylumbelliferyl)-a- D -N-acetylneuraminic acid sodium salt hydrate	pH 7.0	315	374	Reaction product	360	449
4-Methylumbelliferyl N-acetyl-ß- D -glucosaminide dihydrate	pH 10.0	317		Reaction product	360	449
4-Methylumbelliferyl butyrate	MeOH	312		Reaction product	360	449
4-Methylumbelliferyl a- D -glucopyranoside	pH 9.0	317	374	Reaction product	360	449
4-Methylumbelliferyl-ß- D -glucuronide hydrate	pH 9.0	316	375	Reaction product	360	449
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride hydrate	pH 6.0	323	464	Reaction product	360	449
4-Methylumbelliferyl oleate	MeOH	312		Reaction product	360	449
Monochlorobimane	MeOH	380	461	Thiol adduct	380	480
Naphthofluorescein	pH 10.0	594	663
2-Naphthol	0.1 M NaOH	320	410
1-(2-Naphthoyl)imidazole	H2O	230	362	Aldite adducts	234	374
NIR-797 isothiocyanate	MeOH	668	798
12-(7-Nitrobenzofurazan-4-ylamino)dodecanoic acid	MeOH	467	539
6-(7-Nitrobenzofurazan-4-ylamino)hexanoic acid	MeOH	467	539
8-Octadecyloxypyrene-1,3,6-trisulfonic acid trisodium salt	H2O	404	434
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt	H2O	384	409	Reaction product	460	520
Oxonol VI	MeOH	599	634
PBFI-AM	MeOH	369		with Na	334	525
Phenanthridine	DMSO	265	415
3-Phenylumbelliferone	pH 3.0	338	463	pH 10.0	383	472
Phthaldialdehyde	pH 7.0	257		Thiol adduct	334	455
Propidium iodide	pH 7.2	482	608	bound to DNA	540	608
Pyrene	n-Pentane	350	392
1-Pyrenedecanoic acid	MeOH	341	377
1-Pyrenedodecanoic acid	MeOH	341	377
1-Pyrenesulfonic acid sodium salt	MeOH	344	376
Quin-2				with calcium	332	493
Quinine	0.5 M Sulfuric acid	347	448
Quinine hemisulfate salt monohydrate	H2O	343	453
Quinine hydrochloride dihydrate	H2O	322	450
Resorufin acetate	DMSO	540	590	Reaction product	571	585
Resorufin butyrate	DMSO	540	590	Reaction product	571	585
Resorufin ethyl ether	MeOH	464
Resorufin methyl ether	MeOH	463
Rhodamine B	Acidic EtOH	554	627
Rhodamine 6G	MeOH	528	551
Rhodamine 101	MeOH	565	589
Rhodamine 110 chloride	MeOH	499	521
Rhodamine 800	MeOH	528	547
Rhodamine B octadecyl ester perchlorate	MeOH	554	578
Sulforhodamine B acid chloride	H2O	565	586
p-Terphenyl	EtOH	277
Tetramethylrhodamine ethyl ester perchlorate	MeOH	549	574
Tetramethylrhodamine isothiocyanate mixed isomers	MeOH	543	571
meso-Tetraphenylporphyrin	Toluene	645	651
N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate	MeOH	355	450
Umbelliferone	95% EtOH	325	441