The development of near-Infrared (NIR) fluorescent dyes with high fluorescence efficiency and low toxicity is key to the development of NIR fluorescence imaging technology. Alfa Chemistry has the experience to provide guidance in the design and preparation of NIR fluorescent dyes. We can provide a wide range of NIR dyes.
- Cyanines is a class of excellent fluorescent dyestuffs, consisting of a class of conjugated organic small molecule systems comprising an odd number of carbon atoms forming a resonating hypomethyl conjugated chain and capped by two nitrogen-containing heterocyclic rings.
- BODIPY is a collective name for boron difluoride dipyrrole methacrylate dyes and their derivatives, which have the characteristics of high resistance to photobleaching, high quantum yield and insensitivity to the acidity and alkalinity of the environment, and narrow absorption/emission peaks.
- Rhodamine analogs are a class of organic fluorescent dyes consisting of an amino-substituted xanthene parent at positions 3 and 6 with a 9-position carbon atom substituted aryl group.
- Squaraines dyes usually have good photostability and can be produced by nucleophilic substitution reactions of dihydroxycyclobutene diones with electron-rich aryl rings or aryl heterocyclic compounds (e.g., N, N-dialkylaniline, phenols, pyrroles, quinolines).
- Porphyrin dyes are obtained by modifying the porphyrin backbone (metal center and axial ligands), replacing hydrogens with fluorine, and adding different side and anchoring groups.
Figure 1. Near infrared fluorescent dyes that have been used in clinical trials until now.
Key Features
- Near-infrared emission (typically 700-900 nm).
- High brightness.
- Excellent photostability.
- Low background noise.
- Broad compatibility.
Applications
- Biological Imaging - NIR Dyes are widely used in fluorescence microscopy, live-cell imaging, and in vitro and in vivo imaging of biological samples.
- Molecular Diagnostics - These dyes are utilized in molecular diagnostic techniques, such as DNA and RNA detection, protein labeling, and immunoassays.
- Drug Discovery - NIR Dyes play a crucial role in drug discovery research, including high-throughput screening, target identification, and pharmacokinetic studies.
- Bioconjugation - NIR Dyes can be easily conjugated to biomolecules, such as antibodies, peptides, and oligonucleotides, for specific targeting and labeling purposes.
Reference
- Mérian J, et al. (2012). "Fluorescent Nanoprobes Dedicated to in Vivo Imaging: From Preclinical Validations to Clinical Translation." Molecules, 17(5), 5564-5591.
