Fluorescein

• CAS: 2321-07-5
• Catalog Number: ACM2321075
• Category: Other Products
• Molecular Weight: 332.31
• Molecular Formula: C20H12O5
• Description: Alfa Chemistry offers Fluorescein products for various research purposes. Please contact us by email if you do not find the specification you are looking for on this page.
• Synonyms: Acid Yellow 73
• IUPAC Name: 3',6'-dihydroxyspiro[2-benzofuran-3,9'-xanthene]-1-one
• Canonical SMILES: C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
• InChI: InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
• InChI Key: GNBHRKFJIUUOQI-UHFFFAOYSA-N
• Melting Point: 315 °C(dec.)
• Solubility: Insoluble in water Very soluble in Acetone Insoluble in Chloroform,Benzene,Ether
• Appearance: Orange to Amber to Dark red powder to crystal
• Application: Applications of xanthene dyes involve optical materials and organic dyes for medical diagnosis research. Several characteristic features of xanthene dyes are large absorption and luminescence, excellent light resistance, low toxicity in-vivo, and relatively high solubility in water.
• Absorption Wavelength: (max.) 490(0.1mol/L NaOH sol.) nm
• Color/Form: Red, orthorhombic prisms;Yellowish-red to red powder;Orange-red, crystalline powder
• Complexity: 522
• Covalently-Bonded Unit Count: 1
• Decomposition: When heated to decomposition it emits acrid smoke and irritating fumes.
• EC Number: 219-031-8
• Exact Mass: 332.068473g/mol
• Features And Benefits: Both cationic and anionic xanthene dyes are known to be efficient fluorescent dyes. Functional groups on the xanthene moiety control their fluorescent colors.
• Formal Charge: 0
• H-Bond Acceptor: 5
• H-Bond Donor: 2
• Heavy Atom Count: 25
• LogP: log Kow = 3.35 (est);3.4
• Loss On Drying: max. 1.0 %
• MDL Number: MFCD00005050
• Monoisotopic Mass: 332.068473g/mol
• NSC Number: 759114;667256
• Other Experimental: Fluorescein is a fluorescent indicator. From pH 4 to pH 4.5, it changes from colorless to green fluorescent.;Henry's Law constant = 8.9X10-17 atm-cu m/mol at 25 °C (est);Hydroxyl radical reaction rate constant = 2.0X10-10 cu cm/molec-sec at 25 °C (est)
• Physical State: (20 deg.C) Solid
• Rotatable Bond Count: 0
• Storage Conditions: Store at room temperature and dry
• UNII: TPY09G7XIR
• Vapor Pressure: 4.1X10-14 mm Hg at 25 °C (est)
• XLogP3: 3.4

Synthetic Use

Upstream Synthesis Route 1

• 85-44-9
• 108-46-3

• 937-30-4
• 2321-07-5

Reference: [1] Patent: EP2769765, 2014, A1, . Location in patent: Page/Page column

Upstream Synthesis Route 2

• 85-44-9
• 108-46-3

• 2321-07-5

Reference: [1]Cihelnik, Simon; Stibor, Ivan; Lhotak, Pavel
[Collection of Czechoslovak Chemical Communications, 2002, vol. 67, # 12, p. 1779 - 1789]

Downstream Synthesis Route 1

• 2321-07-5

• 15086-94-9

Reference: [1] Patent: US2008/61289, 2008, A1, . Location in patent: Page/Page column 6-7

Downstream Synthesis Route 2

• 67-56-1
• 2321-07-5

• 70672-06-9

Reference: [1]Gitsov, Ivan; Guo, Dandan; Luo, Juntao; Scheibel, Dieter Michael
[Biomacromolecules, 2020, vol. 21, # 6, p. 2132 - 2146]

Downstream Synthesis Route 3

• 108-24-7
• 2321-07-5

• 596-09-8

Reference: [1]Huang, Limin; Wei, Di; Wu, Zibo; Hou, Yajing; Xie, Yitong; Liu, Zhenru; Wang, Cheng; Che, Delu; Lei, Yibo; He, Huaizhen
[Chemical Biology and Drug Design, 2018, vol. 92, # 6, p. 1963 - 1971]

* For details of the synthesis route, please refer to the original source to ensure accuracy.