Stable Cell Line Development Services - Collagen / Alfa Chemistry

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Stable Cell Line Development Services

Stable cell lines have been widely used in recombinant protein and antibody production, drug screening, gene function research and other uses. They can grow continuously for a long time and stably carry transgenic genes or express transgenic genes without significant changes in expression levels. Utilizing our CHO (Chinese Hamster Ovary) and HEK-293 (Human Embryonic Kidney 293) expression vectors, Alfa Chemistry offers a complete package for stable cell line development, or we can start with yours.

Stable Cell Line Development Services

Further assay development and characterization can also be done at our R&D facility, including but not limited to RT-PCR (Reverse Transcription Polymerase Chain Reaction), FACS (Fluorescence Activated Cell Sorting) analysis, western blot, expression stability test, etc. For more technical services, please feel free to contact us.

Service Details

Gene constitutive expression

Single gene overexpression stable cell pool/cell line
Multiple gene overexpression table cell pool/cell line

Service method: viral based & chemical based
Service period: 8+ weeks

Gene inducible expression

Tet-On system
Tet-Off system

Service method: viral based & chemical based
Service period: 12+ weeks

Add-on Services

RT-PCR A laboratory technique combining reverse transcription of RNA into DNA and amplification of specific DNA targets using polymerase chain reaction.
FACS analysis A technique used in flow cytometry to physically separate cells based on their fluorescent properties.
Western blot An analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract.
Expression stability test Validate the GOI expression stability by WB or FACS in 15/20 generation.

Service Workflow

Project discussion

Contract confirmation

Pilot expression & purification studies

Feedback, discussion & sample delivery

Quote for bulk

Scalable production according to customer requirements

NOTE
* All timelines are approximate. In most cases, we deliver within 4-6 weeks.

Possibilities are unlimited with Alfa Chemistry. Let's discuss your idea.

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Please kindly note that our products and services are for research use only.