Mammalian Transient Protein Expression Services

Mammalian Transient Protein Expression Services

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Mammalian Transient Protein Expression Services

Mammalian Transient Protein Expression Services

The production of mammalian proteins has become the standard for the production of state-of-the-art biotherapeutics, as well as becoming routine in the production of protein samples for research purposes. Variables such as cell line, culture media, and culture method affect the yield, physical properties, and biological activity of the expressed proteins.

Alfa Chemistry is a leading expert in the preparation of all types of proteins using our signature mammalian transient expression systems. We specialize in HEK293* and CHO* cells. We also offer flexible additional services to meet customer needs. For more technical services, please feel free to contact us.

Service Details

Service Details

Gene synthesis and codon optimization

  • Only the protein/gene sequence or plasmid is required.

Service period: 1-2 weeks

Vector construction

  • cDNA cloning into expression vector
  • Plasmid sequencing
  • Plasmid preparation

Service period: 1-2 weeks
Deliverables: Sequencing report

Protein expression and purification

  • Transient transfection of HEK293/CHO cells
  • Protein purification
  • QC analysis (SDS-PAGE, UV, etc.)

Service period: 2-3 weeks
Deliverables: 0.1-0.5 mg purified proteins & CoA

Large-scale expression and purification

  • 100+ mg
  • QC analysis

Service period: 3-4 weeks
Deliverables: Purified proteins & CoA

Service Workflow

Project discussion

Project discussion

Contract confirmation

Contract confirmation

Pilot expression and purification studies

Pilot expression & purification studies

Feedback, discussion and sample delivery

Feedback, discussion & sample delivery

Quote for bulk

Quote for bulk

Scalable production according to customer requirements

Scalable production according to customer requirements

NOTE
* HEK 293 (Human Embryonic Kidney 293) cells are commonly used for biopharma and basic medical research and therapeutic solutions because of their ease of transfection, high mammalian protein expression levels and robust workflows.
* CHO (Chinese hamster ovary) cells are an epithelial cell line, often used in biological and medical research and commercially in the production of recombinant therapeutic proteins.
* All timelines are approximate. In most cases, we deliver within 4-6 weeks.

Possibilities are unlimited with Alfa Chemistry. Let's discuss your idea.

Please kindly note that our products and services are for research use only.