Bacterial Protein Expression Services

Bacterial Protein Expression Services

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Bacterial Protein Expression Services

Bacterial Protein Expression Services

Recombinant protein expression in bacteria requires the insertion of a DNA fragment into an expression vector, routinely a plasmid vector and the transferal of this vector into bacterial cells (transformation). The cells are then cultured and induced to express the desired protein. Among the various bacteria, E. coli is a well-characterized protein-producing organism, being the first host to express an approved therapeutic recombinant protein.

Alfa Chemistry's protein expression experts provide customized bacterial expression services with cutting-edge solutions ranging from codon optimization to gene synthesis to small-scale testing and scale-up, together with protein purification and characterization. We also offer flexible additional services to meet customer needs. For more technical services, please feel free to contact us.

Service Details

Service Details

Gene synthesis, cloning, plasmid prep

  • Gene synthesis and Codon optimization
  • Subcloning into appropriate expression vector
  • Plasmid Prep and DNA QC

Service period: ≤ 2 weeks

Protein expression evaluation

  • Transform plasmids into appropriate bacterial expression strain
  • Protein expression evaluation and optimization

Service period: ≤ 1 weeks

Protein purification, Tag removal & refolding

  • Scale up protein expression using optimized conditions
  • Purification to reach desired protein amounts and purity
  • Tag removal and separation of tag-free protein
  • Refolding in case of insoluble protein

Service period: ≤ 1 weeks

QC & delivery

  • Purity analysis and Detection using SDS-PAGE and Western Blot
  • Bradford assay for quantitation
  • Protein delivery

Service period: ≤ 1 weeks

Service Workflow

Project discussion

Project discussion

Contract confirmation

Contract confirmation

Pilot expression and purification studies

Pilot expression & purification studies

Feedback, discussion and sample delivery

Feedback, discussion & sample delivery

Quote for bulk

Quote for bulk

Scalable production according to customer requirements

Scalable production according to customer requirements

NOTE
* All timelines are approximate. In most cases, we deliver within 4-6 weeks.

Possibilities are unlimited with Alfa Chemistry. Let's discuss your idea.

Please kindly note that our products and services are for research use only.