Recombinant Human Collagen Type XVII /COL17

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Recombinant Human Collagen Type XVII /COL17

General Information

Product Name: Recombinant Human Collagen Type XVII / COL17
Catalog: RHC-XVII-001
Purity: ≥ 97%
Applications: WB, SDS-PAGE

Description

Collagen Type XVII / COL17 is a major transmembrane constituent of the epidermal anchoring complex. It has an N-terminal globular head region that localizes to the hemidesmosomal plaque and a C-terminal collagenous tail that projects into the basal lamina which is a structure that would facilitate interactions with extracellular ligands and intracellular linkers to the cytoskeleton. Due to limitations in the standardization and purity of natural collagen sources currently available for such applications, the production of human collagen in recombinant systems such as yeast, insect cells, and plants, has become the major way to produce human collagen.

The technical parameters of recombinant human collagen type XVII / COL17 we supplied are as follows.

Items	Specification
Molecular Weight	21.2 kDa (calculated molecular mass) 23 kDa (SDS-PAGE)
Species	Human
Host	E. coli
Form	Lyophilized powder
Sequence Fragment	Pro184-Pro375
Buffer	Prior to lyophilization: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 0.01% Sarcosyl, 5% Trehalose.
Reconstitution	To keep the original salt concentration, we recommend reconstituting to the original concentration prior to lyophilization (see Concentration) in ddH2O. If a lower concentration is required, dilute in 20 mM Tris, 150 mM NaCl, pH 8.0. If a higher concentration is required, the product can be reconstituted directly in 20 mM Tris, 150 mM NaCl, pH 8.0.
Storage	Store at 2-8 °C for up to one month. Store at -80 °C for up to one year. Repeated freezing and thawing is not recommended.

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