Baculovirus-Insect Cell Protein Expression Services

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Baculovirus-Insect Cell Protein Expression Services

The baculovirus-insect cell expression system is widely used in producing recombinant proteins. This system is particularly well-suited for proteins that are difficult to express in bacteria due to size, complexity, and/or posttranslational processing requirements. Alfa Chemistry's protein expression experts provide customized baculovirus–insect cell protein expression service to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins.

Baculovirus-Insect Cell Protein Expression Services

The cell lines we have available in the facility are Sf9, Sf21 and Hi5 cells. We also offer flexible additional services to meet customer needs. For more technical services, please feel free to contact us.

Service Details

Gene synthesis and subcloning

Subcloning into one or more expression vectors

Service period: ≤ 2 weeks

Virus production and amplification

Generation of recombinant Bacmid DNA
Transfection of insect cell with recombinant Bacmid DNA
Generation of P1 stock (Low titer), P2 stock (High titer), and determination of virus titer by Quantitative-PCR

Service period: 2-3 weeks

Pilot expression evaluation