Alfa Chemistry is dedicated to providing advanced solutions in the field of analytical instrumentation. Our instrument platform for ultraviolet (UV) spectroscopy is designed to offer precise, reliable, and high-throughput analysis for various applications across multiple industries.
What is Ultraviolet Spectroscopy?
UV spectroscopy is a technique used to measure the absorbance of UV light by a sample, providing valuable information about its molecular structure and concentration. The UV spectrum typically ranges from 200 to 400 nm.
This region is divided into:
- Near UV (NUV): 300~400 nm
- Far UV (FUV): 200~300 nm
The fundamental principles underlying UV spectroscopy are based on the interaction of UV light with matter, particularly the electronic transitions that occur in molecules. The primary electronic transitions that occur in UV spectroscopy involve the movement of electrons between molecular orbitals. These include:
- π to π* transitions: Common in molecules with double bonds (e.g., alkenes, aromatics).
- n to π* transitions: Involving non-bonding electrons, typical in compounds with lone pairs (e.g., carbonyls).
This method is particularly useful for analyzing organic compounds, including proteins, nucleic acids, and various industrial chemicals, due to the presence of chromophores that absorb UV light at specific wavelengths.
Our Cutting-Edge Instrument
Our UV-Vis Spectrophotometer is equipped with state-of-the-art components that enable high-performance analysis of samples in the UV-visible range. This instrument utilizes advanced optics and a highly sensitive detector to achieve exceptional spectral resolution and accuracy.
Key Features
- Wavelength Range:190~900 nm.
- Wavelength Accuracy: ± 0.2 nm.
- Spectral Bandwidth: With adjustable spectral bandwidth settings, we can tailor measurements to the specific requirements of different samples.
- Data Accuracy: The instrument ensures precise measurement of absorbance, transmittance, and concentration, crucial for quantitative analysis in research and quality control.
Our Services Offered
Our platform offers a wide range of analytical testing services to meet different research needs:
Quantitative Analysis
- Precise quantification of active pharmaceutical ingredients (APIs), excipients, and other compounds in complex mixtures.
- Concentration determination of nucleic acids and proteins in biological samples.
Qualitative Analysis
- Identification of unknown compounds based on their UV absorption spectra.
- Characterization of complex mixtures to determine the presence of specific substances.
Purity and Contaminant Analysis
- Detection and quantification of impurities and degradation products in pharmaceuticals, chemicals, and food products.
- Analysis of environmental samples for pollutants and contaminants.
Our UV spectroscopy technology platform is designed to provide comprehensive analytical services, support research and development, ensure quality control, and facilitate environmental monitoring. By leveraging our expertise and advanced instrumentation, we deliver reliable, accurate, and timely results that drive innovation and ensure compliance across various industries. For more information on our services or to discuss your specific needs, please contact our team or visit our website.
Reference
- Molecular Fingerprinting of Nanoparticles in Complex Media with Non-contact Photoacoustics: beyond the Light Scattering Limit. Scientific Reports (2018).
