The flash chromatograph is a powerful instrument that plays a crucial role in our company's analytical capabilities. This state-of-the-art system enables efficient separation, purification, and isolation of chemical compounds, making it an essential tool for a wide range of applications.
Introduction to Flash Chromatography
Flash chromatography, also known as medium-pressure chromatography, is designed to separate mixtures of compounds quickly and efficiently, providing researchers with purified samples essential for further analysis and application. The technique employs a combination of solvent and pressure to drive the sample through a column packed with a stationary phase, facilitating the separation of components based on their chemical properties.
Figure 1. A typical flash chromatography setup.
Principles of Flash Chromatography
The underlying principles of flash chromatography are similar to those of traditional column chromatography, but with enhancements that allow for faster processing times and higher resolution separations. Key principles include:
- Stationary Phase - A solid material, typically silica gel or alumina, which acts as the medium through which the sample mixture is passed.
- Mobile Phase - A solvent or mixture of solvents that carries the sample through the stationary phase.
- Pressure Application - Unlike gravity-fed chromatography, flash chromatography utilizes a controlled application of pressure to push the solvent and sample through the column, significantly reducing the time required for separation.
- Separation Mechanism - Compounds in the sample interact differently with the stationary phase based on their polarity, size, and other chemical properties, leading to their separation as they elute at different rates.
Our Cutting-Edge Instruments
Our flash chromatography offers 4 times the purification capacity using multi-column FLASH chromatography, a four-column sequential FLASH purification system with an extended fraction bed, designed for multiple sample purification.
Key features include:
- High-efficiency columns, ensuring maximum resolution and reproducibility.
- Advanced pumping systems, delivering consistent and controllable flow rates, optimizing separation efficiency.
- Automated Sample Injection, reducing manual handling and enhancing reproducibility.
- Versatile detection options, including UV-Vis, ELSD, and MS, enable comprehensive analysis of separated compounds.
Tests Using Our Flash Chromatography Platform
Our flash chromatography platform supports a wide range of applications across a variety of industries. Some of the key tests and analyses that can be performed using our instrument platform include, but are not limited to:
Purification of Compounds
- Isolation of target compounds from complex mixtures.
- Removal of impurities to achieve desired purity levels.
- Separation of reaction products.
Compound Library Generation
- Creation of libraries of pure compounds for high-throughput screening.
- Fractionation of natural products extracts.
Analytical Testing
- Verification of compound purity through subsequent analytical techniques such as NMR, HPLC, or GC.
- Comparison of fractions to known standards to confirm identity.
Compound Isolation for Structural Elucidation
Isolation of compounds for structural analysis using techniques like NMR, MS, and X-ray crystallography.
Synthetic Chemistry Support
- Purification of intermediates and final products in synthetic pathways.
- Removal of by-products and side products from synthetic reactions.
For more information about our flash chromatography platform, services, or to discuss potential collaborations, please contact us.
Reference
- mRNA purification: Technology aspects and impurities. Curr Med Drug Res (2022).
