Tetrahydrocortisone

• CAS: 53-05-4
• Catalog Number: ACM53054-1
• Category: Main Products
• Molecular Weight: 364.48
• Molecular Formula: C21H32O5
• Synonyms: Urocortisone;; Tetrahydro E;; Tetrahydrocompound E
• Boiling Point: 415.66 °C
• Melting Point: 189-192 °C
• Appearance: White to Off-White Solid

Case Study

MO-TMS Derivatization of Tetrahydrocorticoids for GC-MS Analysis

Furuta T, et al. Steroids, 1999, 64(12), 805-811.

The derivatization of tetrahydrocorticoids into methoxime-trimethylsilyl (MO-TMS) derivatives represents a reliable method for their quantitative analysis via gas chromatography-mass spectrometry (GC-MS).
Methodology: Tetrahydrocorticoids (approximately 1 mg) were subjected to a sequential derivatization procedure. Initially, 100 μL of methoxyamine hydrochloride in pyridine (5%, w/v) was added to the residue in a 0.3-mL microproduct V-vial. The reaction mixture was sealed under a nitrogen atmosphere and heated to 100°C for 30 minutes, enabling the formation of methoxime derivatives. Following solvent removal under a nitrogen stream, 50 μL of N,O-bis(trimethylsilyl)acetamide (BSA) was introduced to the residue. The reaction mixture was resealed under nitrogen and heated at 100°C for 2 hours to facilitate trimethylsilylation. Post-reaction, the mixture was cooled, and the solvent was evaporated under nitrogen. A subsequent addition of 200 μL of n-hexane enabled preparation for silica cartridge purification. The solution was passed through a Sep-Pak silica cartridge, eluted with 7 mL of n-hexane. The eluate was evaporated at room temperature under nitrogen to yield the tetrahydrocorticoids as MO-TMS derivatives, suitable for GC-MS analysis.

Custom Q&A

What is the molecular formula of tetrahydrocortisone?

The molecular formula of tetrahydrocortisone is C21H32O5.

What are the synonyms of tetrahydrocortisone?

The synonyms of tetrahydrocortisone include TETRAHYDROCORTISONE, Urocortisone, Ba 2681, and Cortisone, tetrahydro-.

What is the InChIKey of tetrahydrocortisone?

The InChIKey of tetrahydrocortisone is SYGWGHVTLUBCEM-ZIZPXRJBSA-N.

What is the IUPAC name of tetrahydrocortisone?

The IUPAC name of tetrahydrocortisone is (3R,5R,8S,9S,10S,13S,14S,17R)-3,17-dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-2,3,4,5,6,7,8,9,12,14,15,16-dodecahydro-1H-cyclopenta[a]phenanthren-11-one.

What is the molecular weight of tetrahydrocortisone?

The molecular weight of tetrahydrocortisone is 364.5g/mol.

How many hydrogen bond donor counts does tetrahydrocortisone have?

Tetrahydrocortisone has three hydrogen bond donor counts.

How many hydrogen bond acceptor counts does tetrahydrocortisone have?

Tetrahydrocortisone has five hydrogen bond acceptor counts.

How many rotatable bond counts does tetrahydrocortisone have?

Tetrahydrocortisone has two rotatable bond counts.

What is the CAS number of tetrahydrocortisone?

The CAS number of tetrahydrocortisone is 53-05-4.

What is the exact mass of tetrahydrocortisone?

The exact mass of tetrahydrocortisone is 364.22497412g/mol.