IXA4

• CAS: 1185329-96-7
• Catalog Number: ACM1185329967
• Category: Inhibitors
• Molecular Weight: 436.51
• Molecular Formula: C24H28N4O4
• Description: IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 transiently activates protective IRE1/XBP1s signaling in liver without inducing RIDD or TRAF2/JNK signaling. IXA4 treatment improves systemic glucose metabolism and liver insulin action through IRE1-dependent remodeling of the hepatic transcriptome that reduces glucose production and steatosis. IXA4-stimulated IRE1 activation also enhances pancreatic function. Our findings indicate that systemic, transient activation of IRE1/XBP1s signaling engenders multi-tissue benefits that integrate to mitigate obesity-driven metabolic dysfunction.
• Synonyms: IXA4; IXA-4; IXA 4
• IUPAC Name: N-(1-(2-(methyl(2-(p-tolyloxy)ethyl)amino)-2-oxoethyl)-1H-pyrazol-4-yl)-3-phenoxypropanamide
• Canonical SMILES: CC1=CC=C(OCCN(C(CN2N=CC(NC(=O)CCOC3=CC=CC=C3)=C2)=O)C)C=C1
• InChI: InChI=1S/C24H28N4O4/c1-19-8-10-22(11-9-19)32-15-13-27(2)24(30)18-28-17-20(16-25-28)26-23(29)12-14-31-21-6-4-3-5-7-21/h3-11,16-17H,12-15,18H2,1-2H3,(H,26,29)
• InChI Key: ZVSKMVAWWBSNOY-UHFFFAOYSA-N
• Solubility: Soluble in DMSO
• Appearance: Solid powder
• Shelf Life: >2 years if stored properly
• Storage: Dry, dark and at 0-4 °C for short term (days to weeks) or -20 °C for long term (months to years).
• Biological Target: IXA4 is a highly selective, non-toxic IRE1/XBP1s activator. IXA4 (10 μM; 4 hours) selectively upregulates XBP1s mRNA, relative to genes regulated by ATF6 (e.g., BiP) or PERK (e.g., CHOP), in other cell lines including Huh7 and SHSY5Y cells.
• Drug Formulation: To be determined
• Elemental Analysis: C, 66.04; H, 6.47; N, 12.84; O, 14.66
• Exact Mass: 436.2111
• HS Tariff Code: 2934.99.9001
• In Vitro Activity: IXA4 reduced Aβ levels 50% in conditioned media prepared on CHO7PA2 cells expressing the V717F APP (APPV717F) mutant (Fig. 5B). This study confirmed compound-dependent IRE1/XBP1s activation in these cells by qPCR (Extended Data Fig. 7C).; Reference: Nat Chem Biol. 2020 Oct;16(10):1052-1061. https://pubmed.ncbi.nlm.nih.gov/32690944/
• In Vivo Activity: TBD
• Shipping: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
• Stock Solution Storage: 0-4 °C for short term (days to weeks), or -20 °C for long term (months).