4-Aza-dl-leucine dihydrochloride

• CAS: 102029-69-6
• Catalog Number: ACM102029696
• Category: Main Products
• Molecular Weight: 205.08
• Molecular Formula: C₅H₁₂N₂O₂·2HCl
• IUPAC Name: 2-amino-3-(dimethylamino)propanoicacid;dihydrochloride
• Canonical SMILES: CN(C)CC(C(=O)O)N.Cl.Cl
• InChI Key: OZWNGZATZQMSHU-UHFFFAOYSA-N
• Appearance: White to off white powder
• Exact Mass: 204.04300

Case Study

4-Aza-Dl-Leucine Dihydrochloride as A Resistance Screening Compound in Metabolic Engineering

Su H, et al Scientific Reports, 2016, 6(1), 39543.

4-Aza-dl-leucine dihydrochloride (AZL) is a leucine analog that can be used as a resistance screening compound in metabolic engineering studies to obtain compounds with high 3-methyl-1-butanol Yields feedback inhibition of AHAS or IPMS mutants. AZL has been previously used against Bacillus subtilis, Escherichia coli, and Salmonella typhimurium to identify drug-resistant strains.
Mutagenicity Experiment
· Yeast cells were adjusted to pH 6.0 with phosphate buffer solution. The chemical mutagen 1-methyl-3-nitro-1-nitroso-guanidine (NTG) was dissolved in 1 mL of acetone and added to each sample (5 mL).
· Adjust the final concentration of NTG to 500 μg/mL, and then incubate in a constant temperature shaker at 30°C for 15 min. The mutant cells were then collected by centrifugation and washed three times with distilled water.
· 200 μL of resuspended mutant cells were inoculated on yeast nitrogen base (YNB) solid medium containing 20 mg/mL 4-Aza-dl-leucine dihydrochloride (AZL), and then cultured at a constant temperature of 30°C. Incubate in the box for about three days until colonies appear.
· Finally, fermentation experiments were conducted using fermented glucose and duckweed hydrolyzate, and the auxotrophic strain with the highest C4~C5 alcohol concentration was identified.

Custom Q&A

What is the molecular formula of 4-Aza-DL-leucine dihydrochloride?

The molecular formula is C5H14Cl2N2O2.

What are the synonyms for 4-Aza-DL-leucine dihydrochloride?

The synonyms are 34064-27-2, 102029-69-6, 2-Amino-3-(dimethylamino)propanoic acid dihydrochloride, and Dap(me)2 2HCl.

What is the molecular weight of 4-Aza-DL-leucine dihydrochloride?

The molecular weight is 205.08 g/mol.

What is the parent compound of 4-Aza-DL-leucine dihydrochloride?

The parent compound is Azaleucine (CID 303570).

What are the component compounds of 4-Aza-DL-leucine dihydrochloride?

The component compounds are Azaleucine (CID 303570) and Hydrochloric Acid (CID 313).

When was 4-Aza-DL-leucine dihydrochloride created?

It was created on August 8, 2009.

When was 4-Aza-DL-leucine dihydrochloride last modified?

It was last modified on October 21, 2023.

What is the IUPAC name of 4-Aza-DL-leucine dihydrochloride?

The IUPAC name is 2-amino-3-(dimethylamino)propanoic acid;dihydrochloride.

What is the InChI of 4-Aza-DL-leucine dihydrochloride?

The InChI is InChI=1S/C5H12N2O2.2ClH/c1-7(2)3-4(6)5(8)9;;/h4H,3,6H2,1-2H3,(H,8,9);2*1H.

What is the CAS number of 4-Aza-DL-leucine dihydrochloride?

The CAS number is 102029-69-6.