ABT-100

• CAS: 450839-40-4
• Catalog Number: ACM450839404
• Category: Inhibitors
• Molecular Weight: 504.46
• Molecular Formula: C27H19F3N4O3
• Description: ABT-100 is an orally bioavailable farnesyltransferase inhibitor. ABT-100 inhibited proliferation of cells in vitro carrying oncogenic H-Ras (EJ-1 bladder; IC(50) 2.2 nmol/L), Ki-Ras (DLD-1 colon, MDA-MB-231 breast, HCT-116 colon, and MiaPaCa-2 pancreatic; IC(50) range, 3.8-9.2 nmol/L), and wild-type Ras (PC-3 and DU-145; IC(50), 70 and 818 nmol/L, respectively) as well as clonogenic potential. ABT-100 shows 70% to 80% oral bioavailability in mice. ABT-100 regressed EJ-1 tumors (2-12.5 mg/kg/d s.c., every day for 21 days) and showed significant efficacy in DLD-1, LX-1, MiaPaCa-2, or PC-3 tumor-bearing mice (6.25-50 mg/kg/d s.c. once daily or twice daily orally).
• Synonyms: ABT100; ABT 100; ABT-100; A367074; A 367074; A-367074
• IUPAC Name: (S)-6-(2-(4-cyanophenyl)-2-hydroxy-2-(1-methyl-1H-imidazol-5-yl)ethoxy)-4'-(trifluoromethoxy)-[1,1'-biphenyl]-3-carbonitrile
• Canonical SMILES: N#CC1=CC(C2=CC=C(OC(F)(F)F)C=C2)=C(OC[C@](O)(C3=CC=C(C#N)C=C3)C4=CN=CN4C)C=C1
• InChI: InChI=1S/C27H19F3N4O3/c1-34-17-33-15-25(34)26(35,21-7-2-18(13-31)3-8-21)16-36-24-11-4-19(14-32)12-23(24)20-5-9-22(10-6-20)37-27(28,29)30/h2-12,15,17,35H,16H2,1H3/t26-/m0/s1
• InChI Key: HEUVRFNVTLGKMZ-SANMLTNESA-N
• Solubility: Soluble in DMSO, not in water
• Appearance: White solid powder
• Shelf Life: >2 years if stored properly
• Storage: Dry, dark and at 0-4 °C for short term (days to weeks) or -20 °C for long term (months to years).
• Alternative CAS: 450839-40-4 (free base); 852926-14-8 (HCl)
• Biological Target: ABT-100 is a farnesyltransferase inhibitor that inhibits cell proliferation (IC50s of 2.2 nM, 3.8 nM, 5.9 nM, 6.9 nM, 9.2 nM, 70 nM and 818 nM for EJ-1, DLD-1, MDA-MB-231, HCT-116, MiaPaCa-2, PC-3, and DU-145 cells, respectively).
• Drug Formulation: This drug may be formulated in DMSO
• Elemental Analysis: C, 64.28; H, 3.80; F, 11.30; N, 11.11; O, 9.51
• Exact Mass: 504.14093
• HS Tariff Code: 2934.99.9001
• In Vitro Activity: To evaluate the effects of ABT-100 on cell growth, this study used the two liver cancer cell lines, HepG2 and Huh7. Cells were counted after 48 hours of incubation with increasing concentrations of ABT-100 in DMEM with 10% FBS (Fig. 1A) or were treated with ABT-100 (20 μmol/L) and the growth rate was evaluated after 2, 4, and 6 days (Fig. 1B). The growth rate of HepG2 and Huh7 cells treated with ABT-100 was significantly lower in comparison to control cells treated with vehicle alone. These results were further confirmed in a soft agar growth assay (Fig. 1C).; Reference: Clin Cancer Res. 2005 Jun 1;11(11):4266-74. https://pubmed.ncbi.nlm.nih.gov/15930366/
• In Vivo Activity: ABT-100 was given to EJ-1 flank tumor-bearing mice at 12.5 and 6.25 mg/kg/d s.c., every day for 21 days, when the mean tumor volume was 265 mm3. ABT-100 induced regression of these tumors, which was maintained as long as the treatment was continued (Fig. 3A). For the 12.5 and 6.25 mg/kg/d groups, the %ILS values were 223 and 239, respectively (P < 0.001 compared with vehicle-treated groups). In a separate experiment, ABT-100 given at 6.25, 3.125, and 2 mg/kg/d orally twice daily produced similar antitumor activity (data not shown). In addition, ABT-100 given at 12.5, 6.25, and 3.125 s.c., every day for 11 days to scid mice bearing orthotopic EJ-1 bladder xenografts showed dose-dependent reduction in bladder tumor weights.; Reference: Clin Cancer Res. 2005 Apr 15;11(8):3045-54. https://pubmed.ncbi.nlm.nih.gov/15930366/
• Shipping: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
• Stock Solution Storage: 0-4 °C for short term (days to weeks), or -20 °C for long term (months).