6-Dimethyl-4-(N-succinimidyloxycarbonyl)phenyl-10-methyl-acridinium-9-carboxylate methosulfate(dmae-nhs)

• CAS: 115853-74-2
• Catalog Number: ACM115853742
• Category: Main Products
• Molecular Weight: 0
• Molecular Formula: C₂₈H₂₃N₂O₆.CH₃O₄S
• Synonyms: 6-Dimethyl-4-(N-succinimidyloxycarbonyl)phenyl-10-methyl-acridinium-9-carboxylate methosulfate (DMAE-NHS);DMAE-NHS;2,6-DiMethylcarbonylphenyl 10-Methyl-9-acridinecarboxylate 4-NHS Ester Methylsulfate;9-[[4-[[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl]-2,6-dimethylphenoxy]carbonyl]-10-methylacridinium methyl sulfate;2,6-DiMethyl-4-(N-succiniMidyloxycarbonyl)phenyl-10-Methyl-acridiniuM-9-carboxylate Methosulfate;Alkyne-PEG5-NHS ester;Acetylene-PEG5-NHS ester;Alkyne-PEG5-N-hydroxysuccinimidyl ester
• IUPAC Name: 2,6-Dimethyl-4-(N-Succinimidyloxycarbonyl)Phenyl 10-Methyl-Acridini...
• Melting Point: 228-230°C
• Exact Mass: 594.13100

Case Study

DMAE·NHS Labeled Total Thyroxine for Chemiluminescence Immunoassay

Yin, Dongguang, et al. Journal of Immunoassay and Immunochemistry, 2008, 29(3), 257-265.

Total thyroxine (TT4) is labeled with DMAE·NHS [2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-10-methyl-acridinium-9-carboxylate methosulfate] acridinium ester, allowing rapid and sensitive determination of TT4 by chemiluminescence immunoassay (CLIA). In this CLIA procedure, microwells were coated with anti-T4 monoclonal antibody (McAb) and streptavidin (SA) was conjugated with DMAE·NHS. Biotin-N-hydroxysuccinimide (B·NHS) was used to conjugate T4-BSA, and in a competitive reaction with anti-T4 McAb, T4-BSA-B·NHS and the T4 in the standard or sample were involved.
Preparation of DMAE·NHS-SA
· A 2 mL brown glass bottle was used to mix 90 mg SA, 200 mL labeling buffer, and 10.5 mL of the 6.5 mmol/L DMAE·NHS solution. Following a 2-hour incubation at room temperature, the reaction was stopped by adding 100 mL of 10 g/L lysine and allowing it to sit at room temperature for 15 minutes.
· The unreacted DMAE·NHS was isolated from the conjugate of DMAE·NHS-SA using size-exclusion chromatography on a 1×25-cm column, with elution performed using a purifying buffer while monitoring the protein peak at 280 nm with a high-pressure liquid chromatograph.
· Chemiluminescence intensity and protein concentration were assessed on a Multilabel Counter and UV-Visible Spectrophotometer, respectively. Fractions exhibiting high chemiluminescence intensity and protein concentration were combined and preserved at -20°C after the addition of 1% BSA.