Fmoc-ACA-OH

• CAS: 378247-75-7
• Catalog Number: ACM378247757
• Category: Amino Acids
• Molecular Weight: 441.43
• Description: Fmoc-ACA-OH is a useful tool for the SPPS of AMC fluorogenic protease substrates by Fmoc SPPS. Fmoc-ACA-OH must be first loaded onto a Wang-type resin. Any unreacted linker hydroxyls must be capped using acetic anhydride. Following Fmoc removal, the first amino acid should be coupled with HATU/collidine. Insertion of ACA into the middle of a peptide provides fluorogenic substrates for endopeptidases. Treatment of the ACC peptide obtained after TFA cleavage with an aqueous base causes facile decarboxylation and formation of the AMC peptide.
• Synonyms: Fmoc-ACA-OH, 7-N-Fmoc-aminocoumarin-4-acetic acid
• InChI: 1S/C26H19NO6/c28-24(29)11-15-12-25(30)33-23-13-16(9-10-17(15)23)27-26(31)32-14-22-20-7-3-1-5-18(20)19-6-2-4-8-21(19)22/h1-10,12-13,22H,11,14H2,(H,27,31)(H,28,29)
• InChI Key: RCUKLQDGAASIAX-UHFFFAOYSA-N
• Application: Peptide synthesis.
• Assay: ≥95.0% (HPLC); ≥98% (TLC)
• Form: powder
• Functional Group: aromatic amine
• Quality Level: 200
• Reaction Suitability: reaction type: Fmoc solid-phase peptide synthesis
• Storage Temperature: 2-8℃