Acid Blue 127:1

• CAS: 12237-86-4
• Catalog Number: ACM12237864
• Category: Other Products
• Molecular Weight: 844.775
• Molecular Formula: C41H26N4Na2O10S2
• Description: Methane, bis(4-((4-amino-3-sulfo-1-anthraquinonyl)amino)phenyl)-, disodium salt, commonly known as Alamar Blue, is a redox indicator that is widely used in scientific research. It is a water-soluble compound that undergoes a color change from blue to pink when reduced, making it a useful tool for measuring cell viability and metabolic activity.
• IUPAC Name: disodium,1-amino-4-[4-[[4-[(4-amino-9,10-dioxo-3-sulfonatoanthracen-1-yl)amino]phenyl]methyl]anilino]-9,10-dioxoanthracene-2-sulfonate
• InChI Key: RISWUWJFSHOUBJ-UHFFFAOYSA-L
• Appearance: Dark blue powder.
• Application: Alamar Blue is commonly used in scientific research to measure cell viability and metabolic activity. It is particularly useful in the study of cell proliferation, apoptosis, and drug toxicity. Alamar Blue can be used to assess the efficacy of drugs in killing cancer cells or to determine the optimal concentration of a drug for maximum effect.
• Storage: Store at 2-8 ℃
• EC Number: 247-287-0
• Exact Mass: 844.089
• PSA: 275.54
• XLogP3: 8.758

Case Study

Acid Blue 127:1 for Evaluation of Antifouling Properties of Plasma Polymer Films

Hegemann D. Thin Solid Films, 2015, 581, 2-6.

Amino-functionalized a-C:H:N films serve as templates for attaching long-chain fluorocarbon molecules to achieve highly durable stain resistance in textiles with minimal use of fluorocarbons. Acid blue 127:1 dye was used to evaluate the dye uptake of ionic polymer films. Its color intensity corresponds not only to the amount of amino groups produced due to chemical interactions, but also to the pore size that enables the dye to diffuse into the plasmonic polymer membrane.
Dye absorption assessment
· The dyeing of the respective a-C:H:N films deposited on polyester fabrics (which do not react with the used dyestuff, C.I. acid blue 127:1) enables to measure the uptake of dyestuff by observing the color intensity K/S.
· Variation of the NH3/C2H4 ratio clearly influences the dye uptake which correlates well with the obtained film densities.
· Furthermore, it was found that the color intensity scales linearly with the film thickness of nanoporous C:H:N films indicating the penetration of the dye molecules into the plasma polymer.

Application of Alamar Blue in Nanomaterial Safety Testing

Longhin E M, et al. Frontiers in Toxicology, 2022, 4, 981701.

Alamar blue (AB) assays are widely used to study cytotoxicity, cell proliferation, and cell metabolic activity in different areas of toxicology. AB is based on the fluorescent redox indicator resazurin, a blue non-fluorescent compound. After intracellular uptake, oxidized resazurin is reduced to fluorescent resorufin due to the reducing environment of the intracellular cytosol. Resorufin produces bright red fluorescence with an excitation range of 530-570 nm and an emission range of 580-610 nm, which can be quantified (fluorescence intensity) and used as a measure of cell viability.
AB assay for nanomaterial testing
· AB is a reliable test to assess the cytotoxic/proliferative/metabolic response of cells exposed to nanomaterials (NMs).
· AB testing can be applied to adherent and suspension cells, as well as co-cultures and 3D advanced models.
· Nanomaterials used for testing should be appropriately dispersed and characterized.
· For exposure of cells to test substances, technical replicates should be performed in the same experiment.
· Alamar blue incubation: Since the AB assay is very sensitive, precise pipetting in subsequent steps is key to obtaining reliable results.
· At the end of the incubation time, the AB signal can be read as absorbance or fluorescence with a spectrometer or fluorometer, respectively.