Oracet blue

Name: Oracet blue
SKU: ACM12769163
Rating: 5 (1 reviews)

CAS

12769-16-3

Catalog Number

ACM12769163

Category

Main Products

Molecular Weight

328.36

Molecular Formula

C₂₁H₁₆N₂O₂

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Specification

Synonyms

LABOTEST-BB LT00847534;ORACET BLUE;ORACET BLUE B;ORACET BLUE B FOR MICROSCOPY;C.I. Solvent Blue 19;1-Anilino-4-(methylamino)anthraquinone,Disperseblue24,Solventblue19;Blue BZL;Solvent blue 19

IUPAC Name

1-anilino-4-(methylamino)anthracene-9,10-dione

Canonical SMILES

CNC1=C2C(=C(C=C1)NC3=CC=CC=C3)C(=O)C4=CC=CC=C4C2=O

InChI Key

TVBNRFCUTVWHQB-UHFFFAOYSA-N

EC Number

221-667-6

Exact Mass

328.12100

Oracet Blue-Based Electrochemical DNA Biosensor for Detection of Helicobacter Pylori

Hajihosseini, Saeedeh, et al. International journal of biological macromolecules, 2016, 91, 911-917.

A DNA electrochemical biosensor based on Oracet Blue (OB) as an electroactive marker was developed for detection of Helicobacter pylori. The results showed that under optimal conditions, the electrochemical signal of the sensor was linear with the target DNA concentration in the range of 0.3 nmolL-1~240.0nmolL-1, with a detection limit of 0.17 nmolL-1 and good reproducibility.
Preparation procedure of the OB-based sensor
· The AuE surface was first polished with alumina-water slurry, rinsed, and dried. A droplet of immobilization buffer with H. pylori probe was deposited on the AuE and left to self-assemble for 105 min at room temperature. The electrode was then washed and incubated in MCH solution for 5 min, followed by rinsing in ethanol-water and distilled water. The hybridization process was carried out by immersing the ss-DNA/AuE in a hybridization buffer with the target oligonucleotide for 120 min, resulting in dsDNA/AuE formation.
· OB was accumulated on the dsDNA/AuE by immersing it in phosphate buffer with OB for 90 min without applying any potential while stirring. The electrode was then rinsed with washing solution. A similar procedure was applied to the accumulation of OB on a bare AuE electrode.

Oracet Blue Modified Screen-Printed Electrodes for Determination of Streptomycin and Oxytetracycline

Akbarzadeh, Sanaz, et al. Biosensors, 2020, 10(3), 23.

The OB/SNPs/GO/SPE modified electrodes prepared by oracet blue, silver nanoparticles and graphene oxide modified screen-printed electrodes (SPE) can be used for determination of streptomycin (STR) in the presence of oxytetracycline (OTC) in milk samples.
Preparation of OB/SNPs/GO/SPE electrodes
· To create the GO/SPE combination for the working electrode, a 2.5 µL droplet of dispersed graphene oxide (GO) at a concentration of 1mg/mL in H2O was applied onto the bare SPE.
· Subsequently, for the preparation of SNPs/GO/SPE, a solution of 100 mM nitric acid and 1.0 mM AgNO3 was deposited onto the GO/SPE. This was followed by a cyclic potential cycle ranging from -700 to 1900 mV at a scan rate of 80 mV s-1 for eight cycles. The modified electrode was then rinsed with distilled water.
· The oracet blue SNPs modified GO/SPE (OB/SNPs/GO/SPE) was created by applying a 2.5 µL droplet of 0.1 mol L-1 phosphate buffer with a pH of 7.0 containing 1.0 mmol L-1 of OB onto the SNPs/GO/SPE surface. OB was immobilized through 18 potential cycles ranging from 0.0 to 230 mV at a rate of 25 mV s-1.
· To produce OB modified GO/SPE (OB/GO/SPE), the GO/SPE was modified following the same procedure but without the addition of SNPs, and the modified electrodes were washed with distilled water.

Study on Modified Glassy Carbon Electrode Containing Oleacetic Acid Blue (OB)

Proposed electrocatalytic reaction of DA at an OBMGCE in low pHs

Zare, Hamid R., et al. Journal of Electroanalytical Chemistry 589.1 (2006): 60-69.

A stable modified glassy carbon electrode containing oleacetic acid blue (OB) was prepared and its electrochemical behavior was studied by cyclic voltammetry. The performance of the electrodeposited film during the preparation process under different conditions and the stability of the deposited film were studied. The charge transfer coefficient a and charge transfer rate constant k for electron transfer between OB and glassy carbon electrode were calculated to be 0.56 and 45.98 ± 0.78 s, respectively. The modified electrode showed electrocatalytic activity for dopamine (DA) oxidation in phosphate buffer solution (pH 8.0) with a decrease in overpotential of about 220 mV and an increase in peak current. The kinetic parameters of DA oxidation on the OBMGCE surface, such as the electron transfer coefficient a, multiphase rate constant k, and standard multiphase rate constant k, were determined using various electrochemical methods. OBMGCE was applied to the electrocatalytic oxidation of dopamine (DA), ascorbic acid (AA), and uric acid (UA), and the overlap of the anodic peaks DA, AA, and UA in differential pulse voltammetry was resolved into three clear voltammetric peaks (DPV). This modified electrode not only can detect DA, AA, and UA very efficiently, but also can simultaneously determine these substances in a mixture. In addition, the modified electrode exhibited stable and sensitive responses to individual solutions of DA, AA, and UA. The calibration curves of DA, AA, and UA were linear for various concentrations of each species.
The reference electrodes were acetate blue modified glassy carbon electrode (OBMGCE), graphite electrode, and saturated calomel electrode (SCE), respectively. All potentials were reported relative to this reference electrode. Electrochemical measurements were performed at laboratory temperature (25 ± 1 °C) using a potentiostat/galvanostat model. pH measurements were performed using a pH/mV meter. Phosphate buffer solution (0.1 M) was prepared from 0.1 M HPO-NaHPO, and the pH was adjusted with 0.1 M HPO or 2.0 M NaOH. DA, AA, and UA solutions were freshly prepared and used. Prior to the electrochemical experiments, all tested solutions were degassed by bubbling with high-purity nitrogen, and a continuous nitrogen flow was maintained over the sample solutions during the experiments.

Application of Oracet Blue for detection of microRNA

Schematic illustration of the biosensor preparation for miRNA detection.

Azimzadeh, Mostafa, et al. Analytical Methods 7.22 (2015): 9495-9503.

MicroRNA in serum/plasma has been introduced as a novel, reliable and specific biomarker for disease detection, condition monitoring and population screening. Together with molecular biology techniques, electrochemical biosensors have become the main biosensing method for miRNA quantification. Studies have attempted to develop an electrochemical biosensing system that is simple, easy to prepare, highly sensitive and selective, cost-effective, and does not require sample preparation and/or amplification. The anthraquinone compound Oracet Blue (OB) was evaluated as an insertable electroactive label for electrochemical biosensing of miRNA. The proposed electrochemical biosensor is made of thiolated single-stranded capture probes (SH-modified SS-probes) on a gold electrode (AuE). The role of OB is to perform electrochemical signal transduction when the SS-Probe hybridizes with the target miRNA. Under optimized conditions, the detection range of the target miRNA was 50 pM to 15 nM, with a detection limit of 13.5 pM. The biosensor can clearly distinguish the target miRNA from single-base mismatched and non-complementary target oligonucleotides, which can guarantee high selectivity and specificity. In addition, the real sample detection results of the proposed biosensor in human serum showed good recovery and high reproducibility, which is expected to play a potential role in clinical applications.
The immobilization buffer was 1.0 M phosphate buffer (pH 4.5), in which the washing and hybridization solution was 0.05 M phosphate buffer (pH 7.0) containing 0.3 M NaCl. Oracet blue solution (0.15mM) was prepared by adding Oracet blue powder to 42.0mL methanol to dissolve and adding 8.0mL phosphate buffer solution (pH 7.0). The resulting modified electrode was placed in the target miRNA solution of the desired concentration for 120 minutes and then gently rinsed with the washing solution. Then, it was immersed in the OB solution for 60 minutes with very slow rotation on a stirrer to better accumulate on the probe-miRNA hybrid assumed by embedding. The reduction signal of OB was measured by DPV with a 25 mV modulation amplitude scan from +0.23 to 0.0 V and a step potential of 0.0049 in PBS buffer (pH 7.0). To provide a control, the biosensor was immersed in OB solution immediately after preparation without hybridization with the target miRNA, and the resulting DPV voltammogram was compared with the voltammogram of the target hybridized biosensor.

What is the molecular formula of Oracet blue?

The molecular formula of Oracet blue is C21H16N2O2.

What are the synonyms of Oracet blue?

The synonyms of Oracet blue are 12769-16-3, Solvent blue 19, and 3179-96-2.

What is the molecular weight of Oracet blue?

The molecular weight of Oracet blue is 328.4 g/mol.

When was Oracet blue created?

Oracet blue was created on August 8, 2005.

When was Oracet blue last modified?

Oracet blue was last modified on October 21, 2023.

What is the IUPAC name of Oracet blue?

The IUPAC name of Oracet blue is 1-anilino-4-(methylamino)anthracene-9,10-dione.