Original Article:
Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway
Juan C. Castro, et al.
Front Plant Sci. 2023, 14, 1099829.
10.3389/fpls.2023.1099829
Vitamin C (L-ascorbic acid, AsA) is an essential compound with pleiotropic functions in many organisms. Past researches have found that the L-galactose pathway is the main pathway regulating AsA biosynthesis in plants. This article focuses on the genetic and biochemical strategies used by plant cells to regulate AsA biosynthesis through the L-galactose pathway.
AsA has multiple biosynthetic pathways in plants, among which the L-galactose pathway was first proposed in 1998. As shown in the figure, the L-galactose pathway for AsA biosynthesis, which converts D-fructose 6-phosphate into AsA, is best supported by multiple genetic and biochemical studies. In this biosynthetic pathway, eight enzymes are involved, including phosphomannose isomerase (PMI: EC 5.3.1.8), phosphomannomutase (PMM: EC 5.4.2.8), GDP-D-mannose pyrophosphorylase (GMP: EC 2.7.7.13), GDP-D-mannose 3',5'-epimerase (GME: EC 5.1.3.18), GDP-L-galactose phosphorylase (GGP: EC 2.7.7.69), L-galactose-1-phosphate phosphatase (GPP: EC 3.1. 3.25), L-galactose dehydrogenase (GDH: EC 1.1.1.117), and L-galactono-1,4-lactone dehydrogenase (GLDH: EC 1.3.2.3).
The AsA content in plant tissues arises from a homeostasis controlled by complex and poorly understood regulatory mechanisms. This work provides a systematic summary and introduction to help researchers gain a deeper understanding of plant genetic and biochemical regulatory strategies.
Chemicals Related in the Paper:
| Catalog Number | Product Name | Structure | CAS Number | Price |
|---|---|---|---|---|
| ACM15572799 | L-Galactose |  |
15572-79-9 | Price |
