Original Article:
The influence of sodium phosphate buffer on the stability of various proteins: Insights into protein-buffer interactions
Pannuru Pavani, et al.
Journal of Molecular Liquids, 2021, 331, 115753.
10.1016/j.molliq.2021.115753
It is well known that proteins are ubiquitous molecules that require certain external environmental conditions to function properly, such as pH, temperature, ionic strength, polarity and external pressure. Among them, solution pH is one of the most important external factors, which is specific to protein structure stability. Finding the right solvent conditions for proteins has been one of the most asked and puzzled questions in the scientific community for many years. Sodium phosphate buffer (PBS) solution is a promising buffer solution in biological research and biochemical processes. Phosphate buffer system is one of the acid-base buffer systems of extracellular fluid in organisms, and its advantages include:
> The pair of H2PO-4/HPO4-2 is capable of buffering in a particular pH range.
> The concentration of phosphate ions in the body varies in different types of biological medium.
> This buffer usually helps maintain a pH around 7.0.
> PBS buffer not only regulates pH changes but also stabilizes proteins. The use of PBS buffer is said to stabilize the protein for long-term storage and biological experiments.
In this work, the stability and structural integrity of four different proteins, lysozyme (Lys), bromelain (BM), hemoglobin (Hb) and human serum albumin (HSA), were investigated in PBS buffer at different concentrations sexual influence. The obtained results show that Lys, BM and HSA are stable in the presence of PBS buffer. This confirms the potential of PBS buffer as a protein/enzyme protectant.
Chemicals Related in the Paper:
| Catalog Number | Product Name | Structure | CAS Number | Price |
|---|---|---|---|---|
| ACM7601549 | Sodium orthohosphate |  |
7601-54-9 | Price |
