Penteado F G
The nanotechnology claims to be a universal technological revolution. The manganese iron oxide (MnFe2O4) is among the nanotechnological materials in focus, due to its unique magnetic and electric characteristics, such as superparamagnetism. Even though, like any other new technology, its potential benefits may be accompanied by hazards and risks of anthropogenic and occupational exposure to this class of nanomateriais. In order to assess its cytotoxic and genotoxic potential in this scenario, and to ascertain if factors such as concentration, time of exposure, synthesis route and coating used to stabilize the colloid would impact the toxicity, manganese iron oxide in 5 forms of presentation were challenged in a battery of 3 in vitro tests in cultures of a cell line representative of the inhalatory route of exposure (immortalized human bronchial epithelia _ BEAS-2B); they were: the luminescent cell viability assay, based on the quantification of adenosine triphosphate (25.6 up to 256 µg.mLfor 6, 24 and 48 hours), the damage to the deoxyribonucleic acid, by the comet assay (25.6 up to 256 µg.mLfor 6 and 24 hours) and the chromosomal fragmentation (clastogenesis) or loss of whole chromosomes (aneuploidy), by the micronucleus test (12.8 up to 256 µg.mLfor 48 hours, and late cotreatment with cytochalasin B, 6 hours after the beginning of exposure). The essential parameters as well as the behavior of the nanoparticles in the cell culture medium were characterized; all nanoparticles were uptaked by cells within 6 hours of treatment; none of the nanoparticles synthesized by coprecipitation, coated with citrate (MnFe2O4-CpCit) or meso-2,3-dimercaptossuccinic acid (MnFe2O4-CpDmsa), and none of those synthesized by thermal decomposition, and coated with citrate (MnFe2O4-TdCit) or meso-2,3- dimercaptossuccinic acid (MnFe2O4-TdDmsa), did cause any statistically or toxicologically significant cytotoxic or genotoxic effect in the concentration range up to 153.6 µg.mLand up to 48 hours of exposure. This is the first in vitro comparative research to address the genotoxicity of manganese iron oxide nanoparticles, with the merit of a comparative factorial analysis, which pointed out that cytotoxicity is dictated by concentration, time and coating; in the other hand, regarding the genotoxicity end points, the time of exposure was a statistically and biologically significant factor for the damage to the deoxyribonucleic acid caused by both citrate-coated nanoparticles, independently of the synthesis route.
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| Catalog Number | Product Name | Structure | CAS Number | Price |
|---|---|---|---|---|
| ACM12063104 | Manganese diiron oxide |  |
12063-10-4 | Price |
| ACM12063104-1 | Magnesium Zinc Iron Oxide Nanoparticle Dispersion | |
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| ACM12063104-2 | Manganese Iron Oxide Nanoparticle Dispersion | |
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