Extraction and Quantification of Verbascoside from Plant Sources

Verbascoside, also known as acteoside, is a natural compound found in a variety of medicinal plants. It is essentially a phenylethanol glycoside composed of caffeic acid, hydroxytyrosol, glucose, and rhamnose, and possesses a variety of bioactive properties, including anti-inflammatory, antibacterial, antiviral, antitumor, antioxidant, analgesic, neuroprotective, immunomodulatory, and cellular memory-enhancing properties.

Due to its multiple bioactive properties, it has significant therapeutic potential. Recent studies have shown that verbascoside has neuroprotective activity in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. verbascoside has important applications in the fields of pharmaceuticals and nutritional foods.

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CAS NO.	Product Name
22323-52-0	Verbascoside

When synthesizing trigonelloside by chemical method, there are problems such as a long synthesis route, many side reactions, tedious chiral splitting, low yield, and high cost, so the trigonelloside from plant extraction source still occupies the dominant position in the market.

Experimental Materials

• Fresh or dried plant material containing verbascoside
• Liquid nitrogen (for fresh material)
• Methanol (HPLC grade)
• Ethyl acetate (HPLC grade)
• Acetonitrile (HPLC grade)
• Water (HPLC grade)
• Sodium carbonate (Na₂CO₃)
• Anhydrous sodium sulfate (Na₂SO₄)
• Silica gel (for purification)

Procedure for The Extraction and Purification of Verbascoside

Sample Preparation:

• If using fresh plant material, freeze it in liquid nitrogen and grind to a fine powder using a mortar and pestle.
• If using dried plant material, grind it to a fine powder using a mortar and pestle.

Extraction:

• Weigh 10 grams of the prepared plant material and transfer it to a conical flask.
• Add 100 mL of methanol to the flask and seal it tightly.
• Place the flask in an ultrasonic bath and sonicate for 30 minutes at room temperature.
• Filter the extract through a filter paper or a syringe filter to obtain a clear solution.

Purification:

• Transfer the filtered extract to a separating funnel.
• Add an equal volume of ethyl acetate to the separating funnel.
• Shake vigorously for several minutes to ensure efficient partitioning.
• Allow the layers to separate, and carefully drain the lower ethyl acetate layer into a separate container.
• Repeat the extraction with ethyl acetate twice more to ensure maximum recovery.

Quantification of Verbascoside

Verbascoside is usually quantified using high-performance liquid chromatography (HPLC) analysis: Prepare a standard solution of verbascoside by dissolving a known amount of verbascoside in methanol to obtain a desired concentration range. Inject the standard solution and the purified sample extract into an HPLC system equipped with a suitable column and detector (e.g., C18 column and UV detector). Set the HPLC conditions, including the mobile phase composition, flow rate, and detection wavelength, optimized for verbascoside analysis. Inject a defined volume of the sample extract into the HPLC system and record the peak area or height. Calculate the verbascoside concentration in the sample using the calibration curve generated from the standard solutions.