Rhodamine 800

• CAS: 137993-41-0
• Catalog Number: ACM137993410
• Category: Main Products
• Molecular Weight: 495.96
• Molecular Formula: C26H26ClN3O5
• Description: Alfa Chemistry offers Rhodamine 800 products for various research purposes. Please contact us by email if you do not find the specification you are looking for on this page.
• Synonyms: 9-Cyano-2,3,6,7,12,13,16,17-octahydro-1H,5H,11H,15H-xantheno[2,3,4-ij:5,6,7-i'j']diquinolizin-18-iumperchlorate;(101027-54-7)rhodamine800
• IUPAC Name: 3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaene-16-carbonitrile;perchlorate
• Canonical SMILES: C1CC2=CC3=C(C4=C2N(C1)CCC4)OC5=C6CCC[N+]7=C6C(=CC5=C3C#N)CCC7.[O-]Cl(=O)(=O)=O
• InChI: InChI=1S/C26H26N3O.ClHO4/c27-15-22-20-13-16-5-1-9-28-11-3-7-18(23(16)28)25(20)30-26-19-8-4-12-29-10-2-6-17(24(19)29)14-21(22)26;2-1(3,4)5/h13-14H,1-12H2;(H,2,3,4,5)/q+1;/p-1
• InChI Key: HACOCUMLBPNDIN-UHFFFAOYSA-M
• Appearance: Purple to Dark purple to Brown powder to crystal
• Application: Applications of xanthene dyes involve optical materials and organic dyes for medical diagnosis research. Several characteristic features of xanthene dyes are large absorption and luminescence, excellent light resistance, low toxicity in-vivo, and relatively high solubility in water.
• Storage: -20 °C in the dark
• Absorbance: (E1%1cm) min. 2000(MeOH, 677 to 681 nm)
• Absorption Wavelength: (max.) 679(MeOH) nm
• Complexity: 1020
• Covalently-Bonded Unit Count: 2
• Emission Maximum: 704 nm
• Exact Mass: 495.156099g/mol
• Excitation Maximum: 682 nm
• Features And Benefits: Both cationic and anionic xanthene dyes are known to be efficient fluorescent dyes. Functional groups on the xanthene moiety control their fluorescent colors.
• Formal Charge: 0
• H-Bond Acceptor: 7
• H-Bond Donor: 0
• Heavy Atom Count: 35
• MDL Number: MFCD00467020
• Monoisotopic Mass: 495.156099g/mol
• Physical State: (20 deg.C) Solid
• Rotatable Bond Count: 0
• Storage Conditions: Store at room temperature and dry

Case Study

Rhodamine 800 as a Reference Substance for Fluorescence Quantum Yield Measurements

Alessi A, et al. Journal of luminescence, 2013, 134, 385-389.

Measuring the fluorescence quantum yield (Φf) of deep red dyes that emit between 635-900 nm presents challenges due to the absence of appropriate standards. This study confirms the viability of Rhodamine 800, a flavonoid dye, as a reference material for fluorescence quantum yields within the deep red emission spectrum. Rhodamine 800 (Rho800) demonstrates excellent photostability, minimal anisotropy, and a satisfactory fluorescence quantum yield.
Experimental methods and results
The quantum yield of rhodamine 800 in pure ethanol has been investigated through a relative approach utilizing cresyl violet (CV) and rhodamine 101 (Rho101) as reference standards, as well as through an absolute fluorometric technique involving measurements taken with an integrating sphere.
The molar absorption coefficient, ε(λ), for Rho800 at 682 nm is measured to be 113,302±6001 M-1 cm-1, while the natural lifetime is recorded at 1.93 ns. The fluorescence quantum yields for Rho800, obtained through two independent methods, show strong correlation, with the maximum differences falling within the calculated uncertainties. For reference, literature values are included in the same table; notably, the fluorescence quantum yields determined through both methods for Rho800 are considerably higher than those reported in the literature.

Fluorescence Properties of Rhodamine 800 Dye in Aqueous Solutions and Polymer Films

Le K Q, et al. Journal of Electronic Materials, 2018, 47(8), 4813-4817.

The photoluminescence (PL) or fluorescence properties of Rhodamine 800 (Rho800) dye formed in aqueous solutions and polymer films were studied. The results show that while the solvent has a weak effect on the PL in terms of intensity and peak wavelength position, the concentration has a significant effect on the luminescence. The optimum PL intensity was observed at a dye concentration of around 10^-5M. In addition, the PL intensity of the dye in film form was lower than that in aqueous solution form, while the peak wavelength was red-shifted due to the presence of PVA.
Preparation procedure of samples
· In the first experiment examining the solvent effect on dye solutions, the dye powder was dissolved in three organic solvents: ethanol, methanol, and cyclopentanol, all at a consistent dye mole fraction of 1.9 × 10^-5 M. To investigate the impact of concentration, dye solutions were prepared in methanol across the entire concentration spectrum, from highly concentrated to very dilute solutions. At elevated dye concentrations, a quenching effect, which can dampen luminescence or fluorescence, might occur.
· The second experiment focused on dye-doped polymer thin films. A dyemethanol solution with a concentration of 5 × 10^-5 M was combined with a 0.25% wt PVA solution in a 1:1 ratio. This mixture was then applied to a glass substrate using a spin-coater, with an initial speed of 500 rpm for 10 seconds followed by 3000 rpm for 70 seconds. Finally, the sample was baked at 95°C for 1 minute to remove the methanol.

Custom Q&A

What is the molecular formula of Rhodamine 800?

The molecular formula of Rhodamine 800 is C26H26ClN3O5.

What are the synonyms for Rhodamine 800?

The synonyms for Rhodamine 800 are 137993-41-0 and 101027-54-7.

What is the molecular weight of Rhodamine 800?

The molecular weight of Rhodamine 800 is 496.0 g/mol.

What is the IUPAC name of Rhodamine 800?

The IUPAC name of Rhodamine 800 is 3-oxa-23-aza-9-azoniaheptacyclo[17.7.1.15,9.02,17.04,15.023,27.013,28]octacosa-1(27),2(17),4,9(28),13,15,18-heptaene-16-carbonitrile; perchlorate.

What is the InChIKey of Rhodamine 800?

The InChIKey of Rhodamine 800 is HACOCUMLBPNDIN-UHFFFAOYSA-M.

How many hydrogen bond acceptor count does Rhodamine 800 have?

Rhodamine 800 has 7 hydrogen bond acceptor count.

What is the topological polar surface area of Rhodamine 800?

The topological polar surface area of Rhodamine 800 is 114Ų.

How many rotatable bond count does Rhodamine 800 have?

Rhodamine 800 has a rotatable bond count of 0.

Is Rhodamine 800 a covalently-bonded unit?

Yes, Rhodamine 800 is a covalently-bonded unit.

What is the CAS number of Rhodamine 800?

The CAS number of Rhodamine 800 is 101027-54-7.