Specification
Description
2,6-Diaminopimelic acid is produced in bacteria and is a component of cell wall. It is also synthesized by higher plants.
Synonyms
2,6-Diaminoheptanedioic acid
Canonical SMILES
NC(CCCC(N)C(O)=O)C(O)=O
InChI
1S/C7H14N2O4/c8-4(6(10)11)2-1-3-5(9)7(12)13/h4-5H,1-3,8-9H2,(H,10,11)(H,12,13)
InChI Key
GMKMEZVLHJARHF-UHFFFAOYSA-N
Melting Point
~295 ℃(dec.) (lit.)
Application
2,6-Diaminopimelic acid (DAP) has been used:
as a supplement in glucose minimal media for screening Bacillus subtilis(44)
as a component of lysogeny broth (LB) agar for screening E coli transformants(45)
as a standard in thin layer chromatography for the characterization of DAP isoforms from Streptomyces strains(46)
Biochem Physiol Actions
2,6-Diaminopimelic acid is essential for microbial metabolism and is a potential marker for quantification of microbial proteins. It is a sensitive and reliable marker and is majorly quantified using gradient high-performance liquid chromatography (HPLC).
Technique
thin layer chromatography (TLC): suitable