ACID BLACK 48

• CAS: 1328-24-1
• Catalog Number: ACM1328241
• Category: Acid Dyes
• Molecular Weight: 563.51
• Molecular Formula: C28H18N3NaO7S
• Description: Acid black 48, also known as C.I. 20470, is a synthetic dye that belongs to the azo dye group. It is widely used in various industries, including textiles, paper, leather, and food. The dye is known for its excellent coloring properties and is used to impart a black color to various materials.
• Synonyms: c.i.acidblack48;ACID BLACK 48;CI 65005;COOMASSIE(R) GREY 3G;Coomassie? Grey 3G;acid black 48 (coomassie grey);Acid Black?8;9,10-Anthracenedione, 1,1'-iminobis[4-amino-, sulfonated
• IUPAC Name: sodium;1-(4-amino-9,10-dihydroxyanthracen-1-yl)imino-4-iminoanthracene-9,10-dione;sulfite
• Canonical SMILES: C1=CC=C2C(=C1)C(=C3C(=CC=C(C3=C2O)N=C4C=CC(=N)C5=C4C(=O)C6=CC=CC=C6C5=O)N)O.[O-]S(=O)[O-].[Na+]
• InChI: InChI=1S/C28H17N3O4.Na.H2O3S/c29-17-9-11-19(23-21(17)25(32)13-5-1-3-7-15(13)27(23)34)31-20-12-10-18(30)22-24(20)28(35)16-8-4-2-6-14(16)26(22)33;;1-4(2)3/h1-12,29,33,35H,30H2;;(H2,1,2,3)/q;+1;/p-2
• InChI Key: OCUHRSSUHNJNQW-UHFFFAOYSA-L
• Boiling Point: 763.3ºC at 760mmHg
• Melting Point: 275ºC
• Flash Point: 415.4ºC
• Appearance: Black Powder
• Application: Acid black 48 has been extensively used in various scientific research applications, including cell biology, biochemistry, and pharmacology. The dye is used as a tracer to label proteins, nucleic acids, and other biomolecules. It is also used in the study of cellular processes, such as endocytosis and exocytosis.; Acid black 48 has been used to study the uptake and distribution of drugs in cells and tissues, making it a valuable tool in drug discovery and development.
• Storage: Store at 2-8 ℃
• Complexity: 867
• EC Number: 215-518-4
• Exact Mass: 563.076
• Heavy Atom Count: 40
• Hydrogen Bond Acceptor Count: 10
• Hydrogen Bond Donor Count: 3
• Monoisotopic Mass: 563.07631538
• PSA: 228.35
• Rotatable Bond Count: 2
• Topological Polar Surface Area: 219Å2
• Vapor Pressure: 3.32E-23mmHg at 25°C
• XLogP3: 5.1898

Case Study

Acid Black 48 Used in Research on Industrial Wastewater Remediation

M S Álvarez, et al. Bioresour Technol. 2013, 146:689-695.

Acid black 48 (AB48) and reactive black 5 (RB5) are anthraquinone and diazo dyes respectively, which are common dyes in textile polluted wastewater. Using acid black 48 and reactive black 5 as target dyes, a novel repair strategy consisting of sequential biological and physical processes has been proposed. The ability of yellow fever anaerobic Bacillus to decolorize acid black 48 and reactive black 5 was verified.
Decolorization Analysis of Acid Black 48
· 0.07 g/L of dyes (AB48) when used independently and 0.05 and 0.04 g/L when mixed (RB5 and AB48, respectively) were sterilized by filtration through a 20 μm filter prior to the addition to the autoclaved medium, in order to avoid any possible alteration of the chemical structure of the dye.
· The flasks were inoculated (3%) with previously obtained cell pellets, which were then incubated in an orbital shaker at 65 °C and 100 rpm.
· Decolorization was measured spectrophotometrically from 300 to 750 nm, calculated by measuring the area under the plot and expressed in terms of percentage.

A Low-Cost Adsorption Treatment Method for Acid Black 48

Hung Y T. Environmental Research, Engineering and Management, 2020, 76(3), 47.

A low-cost absorbent (including peanut shells and onion skin) has been developed for adsorption and removal of acidic black 48 for acidic black 48 in wastewater. For the binary mixture of acid black 48 and coffee wastewater, the onion peel can treat combined wastewater with a maximum transmittance of 80.1% and 75.6% at low and medium concentrations of combined wastewater. While peanut could achieve transmittance of 69.4% at high concentrations of combined acid black 48 and coffee wastewater.
Preparation and batch experiments of binary dye mixture
· Add 1 g acid black 48 dye to 1000 mL water and prepare dye wastewater reserve. A coffee wastewater stock solution was prepared by weighing and mixing 1 g of ground coffee powder to 1000 mL of water.
· Subsequently, the diluted coffee wastewater was mixed with dye wastewater to create a binary mixture of combined dye and coffee wastewater.
· Adsorption investigations were carried out with 50 mL volume and 50, 100, and 200 mg/L concentration of dye solution in centrifuge bottles. Run protocol for dye, coffee, and adsorbent concentration was developed based on trial and error.

The Biodegradation of Acid Black 48 Dye via Saccharomyces Cerevisiae

Mitter E K, et al. Water, Air, & Soil Pollution, 2013, 224, 1-9.

Acid black 48 dyes can be degraded by Saccharomyces cerevisiae immobilized in activated sugarcane bagasse in aqueous solutions. According to Fourier's transformation of infrared lighting method, the dye molecules treated by S. Cerevisiae may change (biodegradation) and may form new metabolites.
Degradation of acid black 48 dye
· Peaks in the FT-IR spectra allowed the detection of changes in the original dye molecule, resulting in the possible formation of new primary amines and phenolic compounds.
· The possible degradation pathway of the dye acid black 48 after contact with fixed and free wine yeast cells is shown in the figure.
· The formation of these toxic and carcinogenic compounds must be controlled in industrial biodegradation processes involving acid black 48 dye, requiring a longer retention time for the complete degradation of the new metabolites formed.
· The use of S. cerevisiae immobilized in sugarcane bagasse is quite attractive for biodegradation in the treatment of textile effluents, as the degradation process begins to occur within 72 h.

Custom Q&A

What is the molecular formula of ACID BLACK 48?

The molecular formula of ACID BLACK 48 is C28H17N3NaO7S.

What is the molecular weight of ACID BLACK 48?

The molecular weight of ACID BLACK 48 is 562.5 g/mol.

What are the synonyms of ACID BLACK 48?

Some synonyms of ACID BLACK 48 include Sodium;1-(4-amino-9,10-dihydroxyanthracen-1-yl)imino-4-iminoanthracene-9,10-dione;sulfite and AKOS015903824 sodium;1-amino-4-[(4-amino-9,10-dioxoanthracen-1-yl)amino]anthracene-9,10-dione;sulfite.

When was ACID BLACK 48 created and modified?

ACID BLACK 48 was created on January 18, 2019, and modified on December 23, 2023.

What is the IUPAC name of ACID BLACK 48?

The IUPAC name of ACID BLACK 48 is sodium;1-(4-amino-9,10-dihydroxyanthracen-1-yl)imino-4-iminoanthracene-9,10-dione;sulfite.

What is the InChIKey of ACID BLACK 48?

The InChIKey of ACID BLACK 48 is OCUHRSSUHNJNQW-UHFFFAOYSA-L.

What is the Canonical SMILES of ACID BLACK 48?

The Canonical SMILES of ACID BLACK 48 is C1=CC=C2C(=C1)C(=C3C(=CC=C(C3=C2O)N=C4C=CC(=N)C5=C4C(=O)C6=CC=CC=C6C5=O)N)O.[O-]S(=O)[O-].[Na+].

What is the CAS number of ACID BLACK 48?

The CAS number of ACID BLACK 48 is 1328-24-1.

How many hydrogen bond donor counts does ACID BLACK 48 have?

ACID BLACK 48 has 4 hydrogen bond donor counts.

Is the compound canonicalized for ACID BLACK 48?

Yes, the compound is canonicalized for ACID BLACK 48.