Gentamycin sulfate

• CAS: 1405-41-0
• Catalog Number: ACM1405410-1
• Category: Main Products
• Molecular Weight: 1506.8
• Molecular Formula: C₆₀H₁₂₇N₁₅O₂₆S
• Synonyms: GENTAMICIN 2,5-SULFATE HYDRATE;genoptics.o.p.;gentalline;gentamicinsolution;nsc-82261;sch9724;gentamicin solution 10mg/ml sterile*filtered;gentamicin solution cell culture tested
• IUPAC Name: Gentamicin Sulfate
• Boiling Point: 797.6ºC at 760 mmHg
• Melting Point: 218-237°C
• Flash Point: 436.2ºC
• Appearance: powder
• Exact Mass: 1505.88000
• Hazard Statements: Xn
• Safety Description: 23-36/37-45-22-24/25
• Stability: Stable under normal temperatures and pressures.
• Supplemental Hazard Statements: H317-H334
• Symbol: GHS08
• WGK Germany: 2

Case Study

Gentamycin Sulfate Impregnated Gelatin Microspheres for Skin Wounds

Zhu, Qiyu, et al. Polymer Bulletin, 2018, 75, 77-91.

Gentamicin sulfate (GS) was adsorbed on to the prepared gelatin microspheres (GMs) and doped on to porous antibacterial collagen (Coll)/cellulose nanocrystals (CNCs) freeze-dried scaffolds that were used as a promising wound dressing. The in vitro release profile of GS showed that the GS/GMs/Coll-CNCs composite scaffold had a sustained release property. In addition, the composite scaffold had low cytotoxicity to NIH-3T3 cells and had a certain antibacterial effect against Escherichia coli and Staphylococcus aureus.
Preparation of GS/GMs/Coll-CNCs scaffold
· 0.1 g SPAN 80 were added to 100 mL olive oil. They mixed and boiled it in a water bath at 60 °C for 30 minutes. Then a solution of 10% (w/v) gelatin was added to the emulsion, which was stored at 10 °C for 20 minutes. Added cold acetone, and stirred for an hour. GMs were separated by centrifugation and washed with acetone and isopropyl alcohol. After this, GMs were submerged for one day at 4°C in a solution of 2.5 wt% glutaraldehyde. For removal of residual glutaraldehyde that had not reacted, the crude solution was suspended in glycine solution for 3 hours at 25 °C.
· To obtain GS-impregnated GMs, 4 μL of a GS solution (25 mg/mL) was added for every 1 mg of the freeze-dried GMs, which were then stored at 4 °C overnight. Afterward, the composite GMs were freeze-dried for one day to remove any residual solvent.
· The GS/GMs/Coll-CNCs scaffold was created by dispersing the composite GS/GMs in 75% ethanol and incorporating them into the crosslinked Coll-CNCs scaffold, followed by air drying.

Research of Serum Antibody-Gentamicin Sulfate Drug Targeting Escherichia Coli

Dong, Wei, et al. Research in Veterinary Science, 2011, 91(3), e136-e143.

This work made an Escherichia coli targeted drug, conjugated Escherichia coli serum antibody (Ab) and gentamicin sulfate (GM sulfate). The molecules of the drug of interest are joined such that the amino groups of the antibody serum and gentamicin sulfate are hydrogen-bonded to the hydroxyl groups of PEG6000 in a stable complex. When the antibody is co-combined with gentamicin sulfate, the antibody targets effectively, and the in vitro antibacterial capacity of gentamicin sulfate is 1000x increased with high efficacy and safety.
Preparation of GM sulfate-antibody-PEG conjugates
· The extracted antibody was buffered into 50 mM potassium phosphate, 2 mM EDTA solution at pH 7.0. We dissolution gentamycin sulfate in dimethylacetamide (DMA) and added it to the antibody and PEG solution for a final molar ratio of gentamycin sulfate/antibody/PEG of 400:2:9. The reaction was left at 4 °C mixed for 24 hours.
· The reacted antibody was removed following the reaction on a GE Healthcare HiTrap desalting column (G-25) pH 6.0, balanced with 35 mM sodium citrate, 150 mM NaCl and 2 mM EDTA. This was usually 40%-60% of the antibody, the final product was typically over 95% monomeric (by gel filtration and laser light scattering).